Bubbers J E, Henney C S
J Immunol. 1975 Jul;115(1):145-49.
The lysis of DBA/2 mastocytoma cells (P815) by alloimmune C57BL/6 thymus-derived lymphocytes was inhibited by cytochalasin A (CA) and cytochalasin B (CB). Although CB prevented incorporation of glucose into lymphocytes, the effector cell susceptibility to the drug was independent of the hexose content of the culture medium. Indeed, cytolysis occurred in the absence of exogenous hexose. Futhermore CA which, like CB, inhibited the adsorption of effector spleen cell populations onto homologous allogeneic monolyers and prevented the migratory movement of lymphoid cells did so without affecting incorporation of glucose into lymphocytes. These findings suggest that the inhibition of cytolysis by CA and CB is mediated by common inhibitory effects on membrane mobility rather than by affecting glucose uptake.
同种免疫的C57BL/6胸腺来源淋巴细胞对DBA/2肥大细胞瘤细胞（P815）的溶解作用受到细胞松弛素A（CA）和细胞松弛素B（CB）的抑制。尽管CB可阻止葡萄糖掺入淋巴细胞，但效应细胞对该药物的敏感性与培养基中的己糖含量无关。实际上，在没有外源性己糖的情况下也会发生细胞溶解。此外，与CB一样，CA抑制效应脾细胞群体吸附到同源异基因单层细胞上，并阻止淋巴细胞的迁移运动，且不影响葡萄糖掺入淋巴细胞。这些发现表明，CA和CB对细胞溶解的抑制作用是通过对膜流动性的共同抑制作用介导的，而不是通过影响葡萄糖摄取来实现的。
