Yu R, Schryvers A B
Department of Microbiology and Infectious Diseases, University of Calgary, AB, Canada.
Can J Microbiol. 1994 Jul;40(7):532-40. doi: 10.1139/m94-086.
The interaction between ruminant transferrins and receptor proteins on the surface of the ruminant pathogens Pasteurella haemolytica, Haemophilus somnus, Pasteurella multocida, Haemophilus agnii, and Moraxella bovis was evaluated by a combination of binding assays and affinity isolation procedures. Membranes isolated from P. haemolytica, P. multocida, and H. agnii were capable of binding sheep, goat, and cattle transferrins whereas binding by membranes from H. somnus and M. bovis was specific for bovine transferrin. Proteolytically derived bovine transferrin C-lobe was capable of inhibiting the interaction between bovine transferrin and both Tbp1 and Tbp2 from P. haemolytica and M. bovis but only Tbp1 from H. somnus and P. multocida. Proteolytically derived N-lobe inhibited the binding of P. multocida and H. somnus Tbp2 to bovine transferrin and the binding of bovine transferrin to the single receptor protein identified in H. agnii. The implications of these results regarding the nature of the ligand-receptor interaction and similarities of this interaction with ligand-receptor interactions in different species are discussed.
通过结合测定和亲和分离程序相结合的方法，评估了反刍动物转铁蛋白与反刍动物病原体溶血巴斯德氏菌、睡眠嗜血杆菌、多杀巴斯德氏菌、阿氏嗜血杆菌和牛莫拉菌表面受体蛋白之间的相互作用。从溶血巴斯德氏菌、多杀巴斯德氏菌和阿氏嗜血杆菌中分离出的膜能够结合绵羊、山羊和牛的转铁蛋白，而从睡眠嗜血杆菌和牛莫拉菌中分离出的膜对牛转铁蛋白具有特异性结合。经蛋白酶水解得到的牛转铁蛋白C-叶能够抑制牛转铁蛋白与溶血巴斯德氏菌和牛莫拉菌的Tbp1和Tbp2之间的相互作用，但仅能抑制睡眠嗜血杆菌和多杀巴斯德氏菌的Tbp1。经蛋白酶水解得到的N-叶抑制了多杀巴斯德氏菌和睡眠嗜血杆菌Tbp2与牛转铁蛋白的结合以及牛转铁蛋白与阿氏嗜血杆菌中鉴定出的单一受体蛋白的结合。讨论了这些结果对于配体-受体相互作用性质的意义以及这种相互作用与不同物种中配体-受体相互作用的相似性。
