Kamo I, Patel C, Patel N, Friedman H
J Immunol. 1975 Aug;115(2):382-6.
Spleen cells from normal DBA/2 mice pretreated with a soluble factor from mastocytoma cells or from ascitic fluid of mastocytoma-bearing mice were markedly impaired in terms of antibody formation to SRBC in vitro. Such immunosuppression by mastocytoma homogenates or ascitic fluid was reversed when syngeneic T cells activated to SRBC were added to the cultures, but not when peritoneal exudate cells or anti-theta-treated normal splenocytes were used. Activated T cells, as well as normal B lymphocytes prepared from spleens of lethally irradiated mice reconstituted with bone marrow cells, were less sensitive to the immunosuppressive factor than non-activated T cells. The ability of educated T cells to restore immunocompetence of suppressed spleen cells in vitro suggests that the target of the immunosuppressive factor from mastocytoma cells may be non-activated T cells, especially those involved in T cell helper function.
用来自肥大细胞瘤细胞的可溶性因子或来自荷肥大细胞瘤小鼠腹水预处理的正常DBA/2小鼠的脾细胞，在体外对SRBC的抗体形成方面明显受损。当将激活的针对SRBC的同基因T细胞加入培养物中时，肥大细胞瘤匀浆或腹水引起的这种免疫抑制被逆转，但当使用腹腔渗出细胞或抗θ处理的正常脾细胞时则不然。与未激活的T细胞相比，激活的T细胞以及由用骨髓细胞重建的致死性照射小鼠的脾脏制备的正常B淋巴细胞对免疫抑制因子的敏感性较低。受过训练的T细胞在体外恢复受抑制脾细胞免疫能力的能力表明，肥大细胞瘤细胞免疫抑制因子的靶标可能是未激活的T细胞，尤其是那些参与T细胞辅助功能的T细胞。
