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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Grant S L, Phillips P A, Gow C B

Department of Medicine, University of Melbourne, Austin Hospital, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1994 Mar;21(3):243-7. doi: 10.1111/j.1440-1681.1994.tb02506.x.

Epidermal growth factor is a potent mitogen that causes natriuresis, diuresis and inhibition of arginine vasopressin-induced water reabsorption. 2. The aim of this study was to determine any interaction between epidermal growth factor and the V1 (vascular) and/or V2 (antidiuretic) arginine vasopressin receptor subtypes. 3. Radioligand binding displacement assays demonstrated that although arginine vasopressin related peptides displaced both radioligands from renal medullary membranes at low concentrations epidermal growth factor displaced neither. 4. Arginine vasopressin V2 receptor second messenger cyclic adenosine monophosphate (cAMP) production was inhibited by epidermal growth factor (IC50 2 x 10(-7) mol/L) as was sodium fluoride cAMP production but only at much higher concentrations. 5. Therefore the diuretic effect of epidermal growth factor is not via direct antagonism of arginine vasopressin receptors but seems mediated via inhibition of the V2 second messenger system.

表皮生长因子是一种强效有丝分裂原，可引起利钠、利尿，并抑制精氨酸加压素诱导的水重吸收。2. 本研究的目的是确定表皮生长因子与V1（血管）和/或V2（抗利尿）精氨酸加压素受体亚型之间是否存在相互作用。3. 放射性配体结合置换试验表明，虽然精氨酸加压素相关肽在低浓度时能将两种放射性配体从肾髓质膜上置换下来，但表皮生长因子却不能。4. 表皮生长因子（IC50为2×10⁻⁷mol/L）可抑制精氨酸加压素V2受体第二信使环磷酸腺苷（cAMP）的产生，氟化钠产生cAMP的过程也受到抑制，但仅在高得多的浓度下才会如此。5. 因此，表皮生长因子的利尿作用并非通过直接拮抗精氨酸加压素受体，而是似乎通过抑制V2第二信使系统介导。

Grant S L, Phillips P A, Gow C B

Department of Medicine, University of Melbourne, Austin Hospital, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1994 Mar;21(3):243-7. doi: 10.1111/j.1440-1681.1994.tb02506.x.

Epidermal growth factor is a potent mitogen that causes natriuresis, diuresis and inhibition of arginine vasopressin-induced water reabsorption. 2. The aim of this study was to determine any interaction between epidermal growth factor and the V1 (vascular) and/or V2 (antidiuretic) arginine vasopressin receptor subtypes. 3. Radioligand binding displacement assays demonstrated that although arginine vasopressin related peptides displaced both radioligands from renal medullary membranes at low concentrations epidermal growth factor displaced neither. 4. Arginine vasopressin V2 receptor second messenger cyclic adenosine monophosphate (cAMP) production was inhibited by epidermal growth factor (IC50 2 x 10(-7) mol/L) as was sodium fluoride cAMP production but only at much higher concentrations. 5. Therefore the diuretic effect of epidermal growth factor is not via direct antagonism of arginine vasopressin receptors but seems mediated via inhibition of the V2 second messenger system.

表皮生长因子是一种强效有丝分裂原，可引起利钠、利尿，并抑制精氨酸加压素诱导的水重吸收。2. 本研究的目的是确定表皮生长因子与V1（血管）和/或V2（抗利尿）精氨酸加压素受体亚型之间是否存在相互作用。3. 放射性配体结合置换试验表明，虽然精氨酸加压素相关肽在低浓度时能将两种放射性配体从肾髓质膜上置换下来，但表皮生长因子却不能。4. 表皮生长因子（IC50为2×10⁻⁷mol/L）可抑制精氨酸加压素V2受体第二信使环磷酸腺苷（cAMP）的产生，氟化钠产生cAMP的过程也受到抑制，但仅在高得多的浓度下才会如此。5. 因此，表皮生长因子的利尿作用并非通过直接拮抗精氨酸加压素受体，而是似乎通过抑制V2第二信使系统介导。