Mulder H, Lindh A C, Ekblad E, Westermark P, Sundler F
Department of Medical Cell Research, University of Lund, Sweden.
Gastroenterology. 1994 Sep;107(3):712-9. doi: 10.1016/0016-5085(94)90118-x.
BACKGROUND/AIMS: Islet amyloid polypeptide (IAPP) or amylin is a novel islet hormone candidate with a suggested role in the regulation of glucose homeostasis and the pathogenesis of non-insulin dependent diabetes mellitus. Occurrence of IAPP in the gastrointestinal tract of rats and humans has also been shown. The expression of IAPP in the stomach of the rat and mouse and the possible colocalization of IAPP and known gastric hormones were investigated in this study.
In situ hybridization, immunofluorescence, and combined in situ hybridization and immunocytochemistry were used.
IAPP messenger RNA and IAPP-like immunoreactivity were shown in the same endocrine cells in the antrum and fundus of the rat and in the antrum of the mouse. IAPP was expressed in a major population of somatostatin-immunoreactive cells as well as in small populations of gastrin- and peptide YY-immunoreactive cells.
Our results establish the synthesis and storage of IAPP in gastric endocrine cells in the rat and mouse. The extensive colocalization of IAPP with somatostatin and to a lesser extent with gastrin and peptide YY suggests that IAPP may modulate endocrine activity in the gastric mucosa in a paracrine and/or autocrine mode.
背景/目的：胰岛淀粉样多肽（IAPP）或胰淀素是一种新型胰岛激素候选物，在调节葡萄糖稳态及非胰岛素依赖型糖尿病发病机制中可能发挥作用。研究还表明，IAPP存在于大鼠和人类的胃肠道中。本研究对大鼠和小鼠胃中IAPP的表达以及IAPP与已知胃激素的可能共定位情况进行了研究。
采用原位杂交、免疫荧光以及原位杂交与免疫细胞化学相结合的方法。
在大鼠胃窦和胃底以及小鼠胃窦的相同内分泌细胞中显示出IAPP信使核糖核酸和IAPP样免疫反应性。IAPP在大部分生长抑素免疫反应性细胞中表达，同时也在少量胃泌素和肽YY免疫反应性细胞中表达。
我们的研究结果证实了大鼠和小鼠胃内分泌细胞中IAPP的合成与储存。IAPP与生长抑素广泛共定位，与胃泌素和肽YY的共定位程度较低，这表明IAPP可能以旁分泌和/或自分泌方式调节胃黏膜中的内分泌活动。
