Bachrach G, Bar-Nir D, Banai M, Bercovier H
Department of Clinical Microbiology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
FEMS Microbiol Lett. 1994 Jul 15;120(3):237-40. doi: 10.1111/j.1574-6968.1994.tb07039.x.
DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.
对编码布鲁氏菌12 kDa蛋白的基因进行DNA测序后发现，该蛋白为核糖体蛋白L7/L12。羊种布鲁氏菌L7/L12的DNA序列与相应的牛种布鲁氏菌基因序列相同，表明这两种微生物近乎相同。将该蛋白的序列与其他生物的序列进行比较时，发现一些结构域高度保守，尤其是C端，这与N端序列缺乏保守性形成对比。布鲁氏菌核糖体蛋白L7/L12是一种免疫显性抗原这一发现，为解释核糖体疫苗的活性提供了新的理论依据。
