加氏乳杆菌刺激的巨噬细胞中编码IFNα的mRNA的表达
Expression of mRNA encoding IFN alpha in macrophages stimulated with Lactobacillus gasseri.
作者信息
Kitazawa H, Tomioka Y, Matsumura K, Aso H, Mizugaki M, Itoh T, Yamaguchi T
机构信息
Laboratory of Animal Products Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
出版信息
FEMS Microbiol Lett. 1994 Jul 15;120(3):315-21. doi: 10.1111/j.1574-6968.1994.tb07052.x.
The ability of Lactobacillus gasseri, a dairy lactic acid bacterium, to induce interferon (IFN) was investigated in murine macrophage cultures. IFN alpha was substantially induced by some strains of L. gasseri and the titers were the highest at a concentration of 100 micrograms ml-1 of L. gasseri DSM20243T. The expression of mRNA encoding IFN alpha was detected in spleen-macrophages (SP-M phi) and Peyer's patch-adherent cells stimulated with L. gasseri DSM20243T. Actinomycin D and cycloheximide added to SP-M phi cultures showed that the mRNA was synthesized by 0.5 h, and that IFN alpha was produced within 3 to 6 h after the stimulation with L. gasseri DSM20243T. The results support the notion that dairy products containing L. gasseri can be 'physiologically functional foods'.
研究了一种乳源乳酸菌——加氏乳杆菌在小鼠巨噬细胞培养物中诱导干扰素（IFN）的能力。某些加氏乳杆菌菌株可大量诱导产生IFNα，在加氏乳杆菌DSM20243T浓度为100微克/毫升时，效价最高。在用加氏乳杆菌DSM20243T刺激的脾巨噬细胞（SP-M phi）和派伊尔结黏附细胞中检测到了编码IFNα的mRNA表达。添加到SP-M phi培养物中的放线菌素D和环己酰亚胺表明，mRNA在0.5小时内合成，并且在用加氏乳杆菌DSM20243T刺激后3至6小时内产生IFNα。这些结果支持了含有加氏乳杆菌的乳制品可以是“生理功能食品”这一观点。
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