Nicolay K, Laterveer F D, van Heerde W L
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands.
J Bioenerg Biomembr. 1994 Jun;26(3):327-34. doi: 10.1007/BF00763104.
A number of amphipathic peptides were tested for their effects on structural and functional properties of isolated rat liver mitochondria. The peptides included the matrix targeting sequence of subunit IV of (yeast) cytochrome c oxidase. Titration experiments in which the mitochondria were incubated with increasing concentrations of the peptides revealed two major stages in the interaction. First, at low peptide/mitochondria ratios, peptide binding to the outer membrane occurred which was accompanied by gradual lysis of the outer membrane at higher ratios. The latter was deduced from the release of adenylate kinase, the classical marker enzyme of the intermembrane space. Secondly, at still higher peptide/mitochondria ratios, the permeability of the inner membrane progressively increased, as evidenced by measurements of respiratory control and of the membrane potential. Complete uncoupling of respiration seemed to precede dissipation of the membrane potential.
测试了多种两亲性肽对分离的大鼠肝线粒体结构和功能特性的影响。这些肽包括（酵母）细胞色素c氧化酶亚基IV的基质靶向序列。用递增浓度的肽孵育线粒体的滴定实验揭示了相互作用的两个主要阶段。首先，在低肽/线粒体比率下，肽与外膜结合，在较高比率下外膜会逐渐裂解。后者是通过腺苷酸激酶（膜间隙的经典标记酶）的释放推导出来的。其次，在更高的肽/线粒体比率下，内膜的通透性逐渐增加，这通过呼吸控制和膜电位的测量得到证明。呼吸的完全解偶联似乎先于膜电位的耗散。
