Hovius R, Thijssen J, van der Linden P, Nicolay K, de Kruijff B
Institute of Molecular Biology and Medical Biotechnology, Utrecht University, The Netherlands.
FEBS Lett. 1993 Sep 6;330(1):71-6. doi: 10.1016/0014-5793(93)80922-h.
The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.
通过测定各种磷脂类对磷脂酶A2、磷脂酰胆碱特异性转移蛋白的可及池，并使用三硝基苯磺酸进行化学标记，研究了完整大鼠肝线粒体的外膜和衍生的外膜囊泡的磷脂拓扑结构。外膜囊泡是封闭的，具有外翻拓扑结构，推测磷脂酰胆碱、磷脂酰乙醇胺、心磷脂以及磷脂酰肌醇加磷脂酰丝氨酸在外小叶中的定位分别为55%、77%、100%和至少30%。完整线粒体中的外膜似乎具有相似的磷脂分布。
