Ottosson M, Vikman-Adolfsson K, Enerbäck S, Olivecrona G, Björntorp P
Wallenberg Laboratory, Department of Heart and Lung Disease, Sahlgren's Hospital, Gothenburg, Sweden.
J Clin Endocrinol Metab. 1994 Sep;79(3):820-5. doi: 10.1210/jcem.79.3.8077367.
The influence of cortisol, in the presence of insulin, on the regulation of lipoprotein lipase (LPL) activity was studied in human adipose tissue, using a tissue incubation technique. Tissue pieces were preincubated for 3 days in a control medium containing insulin (7175 pmol/L), then incubated for 2 additional days in the control medium with and without cortisol (1000 nmol/L). After the 5 days of incubation, the levels of LPL messenger ribonucleic acid (mRNA), relative LPL synthesis, and LPL activity (total and heparin releasable) were studied. Cortisol exposure for 2 days increased all of the variables related to LPL. The average increase was 2.5-fold for LPL mRNA, 3.0-fold for relative LPL synthesis, 5.2-fold for total LPL activity, and 9.4-fold for heparin-releasable LPL activity compared to that in controls without cortisol. The results confirm previous findings that cortisol, in the presence of insulin, has a marked stimulatory effect on LPL activity in human adipose tissue in vitro. New data have been presented on the mechanisms of cortisol regulation of LPL activity. They involve both an increased level of LPL mRNA, leading to increased relative LPL synthesis, and additional posttranslational regulation.
利用组织孵育技术，研究了在胰岛素存在的情况下，皮质醇对人脂肪组织中脂蛋白脂肪酶（LPL）活性调节的影响。将组织块在含有胰岛素（7175 pmol/L）的对照培养基中预孵育3天，然后在添加和不添加皮质醇（1000 nmol/L）的对照培养基中再孵育2天。孵育5天后，研究LPL信使核糖核酸（mRNA）水平、相对LPL合成以及LPL活性（总活性和肝素可释放活性）。暴露于皮质醇2天增加了所有与LPL相关的变量。与未添加皮质醇的对照相比，LPL mRNA平均增加2.5倍，相对LPL合成增加3.0倍，总LPL活性增加5.2倍，肝素可释放LPL活性增加9.4倍。结果证实了先前的发现，即在胰岛素存在的情况下，皮质醇对体外人脂肪组织中的LPL活性具有显著的刺激作用。本文还提供了关于皮质醇调节LPL活性机制的新数据。这些机制包括LPL mRNA水平升高，导致相对LPL合成增加，以及额外的翻译后调节。
