Hasegawa T, Okuda M, Nomoto K, Yoshikai Y
Laboratory of Germfree Life, Nagoya University School of Medicine, Japan.
Immunopharmacol Immunotoxicol. 1994 May;16(2):191-202. doi: 10.3109/08923979409007090.
Oral administration of a hot water extract of Chlorella vulgaris(CVE)(20mg/mouse, 10 consecutive days) augmented the resistance against an i.p. infection with Listeria monocytogenes in mice. The numbers of bacteria in a CVE-administered group were significantly lower in the peritoneal cavity or spleen than those in a control group. FCM analysis revealed that gamma delta +Thy1.2+ cells in the nonadherent PEC from CVE-administered mice increased more prominently in number at the early stage on day 3 or on day 5 after infection as compared with those in control mice. The increment of gamma delta +Thy1.2+ T cells was also evident in spleen in CVE-administered mice at this stage after infection. The proportion of TCR alpha beta +Thy1.2+ T cells in the nonadherent PEC of a control group increased from 13% on day 0 to 49% at the late stage on day 10 after infection, whereas the proportion of TCR alpha beta +Thy1.2+ T cells in the nonadherent PEC in CVE-administered mice increased to 64% on this stage after infection in association with augmentation of DTH response to Listeria. These results suggest that CVE-administration effectively augment cell-mediated immunity against Listeria through the increment of gamma delta + T cells in the early phase and the increment of alpha beta + T cells in the late phase after listerial infection.
口服普通小球藻热水提取物（CVE）（20mg/小鼠，连续10天）可增强小鼠对腹腔注射单核细胞增生李斯特菌感染的抵抗力。CVE给药组小鼠腹腔或脾脏中的细菌数量明显低于对照组。流式细胞术分析显示，与对照小鼠相比，CVE给药小鼠非贴壁腹腔渗出细胞中的γδ+Thy1.2+细胞在感染后第3天或第5天的早期数量增加更为显著。在感染后的这个阶段，CVE给药小鼠的脾脏中γδ+Thy1.2+T细胞的增加也很明显。对照组非贴壁腹腔渗出细胞中TCRαβ+Thy1.2+T细胞的比例从感染后第0天的13%增加到第10天后期的49%，而CVE给药小鼠非贴壁腹腔渗出细胞中TCRαβ+Thy1.2+T细胞的比例在感染后的这个阶段增加到64%，同时对李斯特菌的迟发型超敏反应增强。这些结果表明，给予CVE可通过在李斯特菌感染早期增加γδ+T细胞和后期增加αβ+T细胞，有效地增强针对李斯特菌的细胞介导免疫。
