Gadenne A S, Strucke R, Dunn D, Wagner M, Bleicher P, Bigby M
Department of Dermatology and Cutaneous Biology Research Center (CBRC), Harvard Medical School, Boston, Massachusetts.
J Invest Dermatol. 1994 Sep;103(3):347-51. doi: 10.1111/1523-1747.ep12394904.
Lichen planus is characterized by a dense infiltrate of T lymphocytes at the dermoepidermal junction. To determine the phenotypic and functional characteristics of the infiltrating lymphocytes, T-cell lines from normal and lesional skin from the same patients with lichen planus were established by culture with interleukin 2 followed by stimulation every 14 d with phytohemagglutinin and irradiated allogeneic feeder cells. Resultant T-cell lines were immunophenotyped by staining with monoclonal antibodies and their reactivity tested by determining their cytolytic activity to selected targets. T-cell lines from 13 lesional and nine normal biopsy specimens were studied. T-cell lines from normal skin were 61% CD4+ and 32% CD8+, whereas lines from lesional skin had significantly fewer CD4+ cells (13%) and more CD8+ cells (62%). T-cell lines from lesional skin contained a distinctive population of gamma delta T cells that was rarely present in lines derived from normal skin. We were able to culture gamma delta T cells out of the lesional skin of 12 of 13 patients. In these 12 patients, lesional T-cell lines were 17% gamma delta+ (range 2% to 47%). Only one T-cell line from normal skin contained significant numbers of gamma delta T cells. The gamma delta population from lesional skin was commonly V delta 1J delta 1+. These results suggest that CD8+ and TCR gamma delta+ T lymphocytes may be involved in the development or the maintenance of lichen planus.
扁平苔藓的特征是在真皮表皮交界处有密集的T淋巴细胞浸润。为了确定浸润淋巴细胞的表型和功能特征，通过用白细胞介素2培养，随后每14天用植物血凝素和辐照的同种异体饲养细胞刺激，建立来自同一扁平苔藓患者正常皮肤和病变皮肤的T细胞系。用单克隆抗体染色对所得T细胞系进行免疫表型分析，并通过测定它们对选定靶标的细胞溶解活性来测试其反应性。研究了来自13个病变活检标本和9个正常活检标本的T细胞系。来自正常皮肤的T细胞系61%为CD4 +，32%为CD8 +，而来自病变皮肤的细胞系中CD4 +细胞明显较少(13%)，CD8 +细胞较多(62%)。来自病变皮肤的T细胞系含有独特的γδT细胞群体，而在来自正常皮肤的细胞系中很少出现。我们能够从13例患者中的12例病变皮肤中培养出γδT细胞。在这12例患者中，病变T细胞系17%为γδ+(范围为2%至47%)。只有一个来自正常皮肤的T细胞系含有大量的γδT细胞。来自病变皮肤的γδ细胞群体通常为Vδ1Jδ1 +。这些结果表明CD8 +和TCRγδ+ T淋巴细胞可能参与扁平苔藓的发生或维持。
