Sato S, Yamamoto T, Isegawa Y, Yamanishi K
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan.
J Gen Virol. 1994 Sep;75 ( Pt 9):2349-54. doi: 10.1099/0022-1317-75-9-2349.
Uracil-DNA glycosylase encoded in many species functions as a DNA repair enzyme that removes uracil residues from DNA. To investigate the potential function of uracil-DNA glycosylase encoded by human herpes-virus 6 (HHV-6), we sequenced a DNA clone (pSTY09), identified an open reading frame of 765 bp and compared the putative amino acid sequence with other uracil-DNA glycosylases, by computer analysis. The amino acid sequence of HHV-6 had similarities to other uracil-DNA glycosylases, with the highest degree of similarity to those of human cytomegalovirus and Epstein-Barr virus. Two strongly conserved regions in uracil-DNA glycosylase of other species also existed in HHV-6. The gene product which was expressed in Escherichia coli demonstrated uracil-DNA glycosylase activity. This is the first report to identify and characterize the uracil-DNA glycosylase gene in HHV-6.
许多物种中编码的尿嘧啶-DNA糖基化酶作为一种DNA修复酶，可从DNA中去除尿嘧啶残基。为了研究人类疱疹病毒6型（HHV-6）编码的尿嘧啶-DNA糖基化酶的潜在功能，我们对一个DNA克隆（pSTY09）进行了测序，确定了一个765 bp的开放阅读框，并通过计算机分析将推定的氨基酸序列与其他尿嘧啶-DNA糖基化酶进行了比较。HHV-6的氨基酸序列与其他尿嘧啶-DNA糖基化酶有相似性，与人类巨细胞病毒和爱泼斯坦-巴尔病毒的相似性最高。HHV-6中也存在其他物种尿嘧啶-DNA糖基化酶中的两个高度保守区域。在大肠杆菌中表达的基因产物表现出尿嘧啶-DNA糖基化酶活性。这是首次鉴定和表征HHV-6中尿嘧啶-DNA糖基化酶基因的报告。
