Fuchs W, Mettenleiter T C
Institute of Molecular and Cellular Virology, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.
J Gen Virol. 1996 Sep;77 ( Pt 9):2221-9. doi: 10.1099/0022-1317-77-9-2221.
We have cloned and sequenced the KpnI L and M restriction fragments of infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) DNA, which are localized adjacently at the right end of the unique long region of the genome. Within the 6930 bp DNA sequence six complete open reading frames (ORFs) were identified. The predicted amino acid sequences of four of them exhibit significant homologies to the UL5 (helicase), UL4, UL3 and UL2 (uracil-DNA glycosylase) genes, which are conserved in similar arrangement in all alphaherpesvirus genomes characterized up to now. A short ORF of 72 codons between UL3 and UL4 of ILTV is positionally homologous to the UL3.5 gene present in the genomes of different members of the Varicellovirus genus of alphaherpesviruses, but not in herpes simplex virus. The predicted ILTV protein encoded upstream of UL2 possesses limited sequence homology to the UL1 gene product of herpesviruses, the structural glycoprotein L. The presence of a N-terminal signal sequence, a conserved N-glycosylation site and two conserved cysteine residues indicates a similar function of the putative ILTV protein. Upstream of the UL1 gene of ILTV the C-terminal part of an additional ORF designated as ULO was identified, which exhibits no significant homology to known herpesvirus genes. RNA analyses indicate the expression of all detected ILTV genes including ULO.
我们克隆并测序了传染性喉气管炎病毒（ILTV，鸡疱疹病毒1型）DNA的KpnI L和M限制性片段，它们位于基因组独特长区域的右端相邻位置。在6930 bp的DNA序列中鉴定出6个完整的开放阅读框（ORF）。其中4个的预测氨基酸序列与UL5（解旋酶）、UL4、UL3和UL2（尿嘧啶-DNA糖基化酶）基因具有显著同源性，在目前已鉴定的所有α疱疹病毒基因组中，这些基因以相似的排列方式保守存在。ILTV的UL3和UL4之间有一个72个密码子的短ORF，在位置上与α疱疹病毒水痘病毒属不同成员基因组中存在的UL3.5基因同源，但在单纯疱疹病毒中不存在。预测的在UL2上游编码的ILTV蛋白与疱疹病毒的UL1基因产物、结构糖蛋白L具有有限的序列同源性。N端信号序列、保守的N-糖基化位点和两个保守的半胱氨酸残基的存在表明推定的ILTV蛋白具有相似的功能。在ILTV的UL1基因上游，鉴定出一个额外的ORF（命名为ULO）的C端部分，它与已知的疱疹病毒基因没有显著同源性。RNA分析表明所有检测到的ILTV基因包括ULO都有表达。
