Mori T, Shin Y S, Okita M, Hirayama N, Miyashita N, Gemma T, Kai C, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
J Gen Virol. 1994 Sep;75 ( Pt 9):2403-8. doi: 10.1099/0022-1317-75-9-2403.
Eight isolates of canine distemper virus (CDV) were obtained from seven dogs suffering from distemper by co-cultivation of their mononuclear cells with a marmoset B lymphoblastoid cell line, B95a. Six of the seven dogs had received one or more vaccinations. All of the isolates readily proliferated in B95a cells, but were not completely neutralized by anti-CDV canine plasma, which had high neutralizing activity against the Onderstepoort laboratory strain of CDV. Furthermore, different reactivities of monoclonal antibodies (MAbs) against CDV were observed between the field isolates and laboratory or vaccine strains of CDV in immunofluorescence studies. Immunoprecipitation analysis using MAbs detected the haemagglutinin protein of each new field isolate as 69K, 75K and 155K forms, and the fusion protein as 64K and 65K forms; the corresponding proteins of the Onderstepoort strain were detected as 75K and 61K proteins respectively. It is apparent from these results that the new field isolates of CDV have very different antigenic properties from the Onderstepoort vaccine strain.
通过将七只患犬瘟热的犬的单核细胞与狨猴B淋巴母细胞系B95a共同培养，获得了八株犬瘟热病毒（CDV）分离株。这七只犬中有六只曾接种过一次或多次疫苗。所有分离株均能在B95a细胞中顺利增殖，但不能被对CDV昂德斯普特实验室株具有高中和活性的抗CDV犬血浆完全中和。此外，在免疫荧光研究中，观察到针对CDV的单克隆抗体（MAb）在野外分离株与CDV实验室株或疫苗株之间具有不同的反应性。使用MAb进行的免疫沉淀分析检测到每个新野外分离株的血凝素蛋白有69K、75K和155K三种形式，融合蛋白有64K和65K两种形式；昂德斯普特株的相应蛋白分别检测为75K和61K蛋白。从这些结果可以明显看出，CDV的新野外分离株与昂德斯普特疫苗株具有非常不同的抗原特性。
