Greene D M, Bondy G S, Azcona-Olivera J I, Pestka J J
Department of Food Science, Michigan State University, East Lansing 48824-1224.
J Toxicol Environ Health. 1994 Sep;43(1):37-50. doi: 10.1080/15287399409531902.
Prolonged dietary exposure of female B6C3F1 mice to the trichothecene vomitoxin results in hyperproduction of immunoglobulin A (IgA) with a concurrent immunopathology that mimics human IgA nephropathy. To assess the role of gender and strain in the mouse model, semipurified AIN-76A diet containing 25 ppm vomitoxin was fed to B6C3F1 male mice and to B6C3F1, BALB/c, C3H/HeN, C3H/HeJ, and C57BL/6 female mice for 8 wk, and immunopathologic indicators of IgA nephropathy were compared to mice fed clean diet. At the cessation of the experiment, all treatment groups weighed less than respective controls. Serum IgA was increased in male and female B6C3F1 mice as well as in C3H/HeJ, C57BL/6, and BALB/c female mice compared to corresponding controls. Serum IgA levels were two- to sixfold higher in B6C3F1 male treatment animals compared to female treatment groups from all strains. In contrast, at wk 8 serum IgG levels were unaffected or decreased, and serum IgM was decreased in all groups at wk 8. There was a trend toward increased IgA production by Peyer's patch (PP) lymphocytes isolated from treatment mice as compared to controls in all groups except the C3H/HeJ mice. Notably, IgA levels were 18-fold higher in B6C3F1 male treatment PP cultures than in B6C3F1 female treatment cultures. Hematuria was significantly greater in treatment mice than respective controls at both wk 4 and 8. Increased mesangial IgA deposition was also detectable in all treatment groups except the C57BL/6 mouse. The results suggested that the male B6C3F1 mouse and the five strains of female mice exhibited many of the immunopathologic effects found in IgA nephropathy and that IgA elevation was more marked in male B6C3F1 than female B6C3F1 mice.
将雌性B6C3F1小鼠长期暴露于单端孢霉烯族毒素呕吐毒素中，会导致免疫球蛋白A（IgA）过度产生，并伴有类似人类IgA肾病的免疫病理学变化。为了评估性别和品系在该小鼠模型中的作用，将含有25 ppm呕吐毒素的半纯化AIN - 76A饲料喂给B6C3F1雄性小鼠以及B6C3F1、BALB/c、C3H/HeN、C3H/HeJ和C57BL/6雌性小鼠8周，并将IgA肾病的免疫病理学指标与喂食清洁饲料的小鼠进行比较。在实验结束时，所有治疗组的体重均低于各自的对照组。与相应对照组相比，雄性和雌性B6C3F1小鼠以及C3H/HeJ、C57BL/6和BALB/c雌性小鼠的血清IgA均升高。与所有品系的雌性治疗组相比，B6C3F1雄性治疗动物的血清IgA水平高出两到六倍。相比之下，在第8周时，血清IgG水平未受影响或降低，所有组的血清IgM在第8周时均降低。除C3H/HeJ小鼠外，所有组中与对照组相比，从治疗小鼠分离的派伊尔结（PP）淋巴细胞产生IgA的趋势增加。值得注意的是，B6C3F1雄性治疗PP培养物中的IgA水平比B6C3F1雌性治疗培养物中的高18倍。在第4周和第8周时，治疗小鼠的血尿均明显高于各自的对照组。除C57BL/6小鼠外，所有治疗组中也可检测到系膜IgA沉积增加。结果表明，雄性B6C3F1小鼠和五种品系的雌性小鼠表现出许多IgA肾病中发现的免疫病理学效应，并且雄性B6C3F1小鼠的IgA升高比雌性B6C3F1小鼠更明显。
