Pestka J J, Dong W, Warner R L, Rasooly L, Bondy G S, Brooks K H
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824.
Food Chem Toxicol. 1990 Jun;28(6):409-20. doi: 10.1016/0278-6915(90)90087-4.
Recent investigations indicate that dietary exposure to the trichothecene vomitoxin increases total and antigen-specific serum immunoglobulin A (IgA) and glomerular IgA accumulation in mice. In this study, the effects of 25 ppm dietary vomitoxin on the histological and lymphocytic profile of component immune organs in the mucosal lymphocyte migratory pathway were evaluated in the B6C3F1 mouse. Vomitoxin administration resulted in marked stimulation of the size and frequency of germinal centres in Peyer's patches, mesenteric lymph nodes and the spleen. A slight increase in the percentage of B cells in the Peyer's patch was observed, although vomitoxin treatment had no effect on the percentage of B cells in the spleen. The percentage of IgA+ cells in Peyer's patches and spleen were approximately twice that of controls at 4, 8 and 12 wk of vomitoxin exposure whereas the percentage of IgG+ cells decreased in these two organs. Exposure to vomitoxin increased the percentage of T cells in Peyer's patches and the spleen. The percentage of CD4+ cells (T helper subset) increased slightly in Peyer's patches and more markedly (30-50%) in the spleen following vomitoxin treatment. Contrastingly, there was only a slight increase in the percentage of CD8+ cells (T cytotoxic/suppressor subset) in the spleens of vomitoxin-treated mice in comparison with controls, and no effect in Peyer's patches. The relative effects of vomitoxin on these two T cells populations was also reflected in increased CD4+: CD8+ ratios in Peyer's patches and spleen. These results are consistent with the hypothesis that dietary vomitoxin modulates normal regulation of the IgA response at the Peyer's patch level and that this is manifested in an altered lymphocyte distribution pattern in both the mucosal and systemic compartment. Notably increased levels of IgA+ and CD4+ cells are indicative of IgA-producing progenitors and T helper subsets, respectively, that in tandem could favour IgA hyperproduction and elevated IgA in serum.
最近的研究表明，饮食中接触单端孢霉烯族呕吐毒素会增加小鼠血清中总免疫球蛋白A（IgA）和抗原特异性IgA以及肾小球IgA的积累。在本研究中，在B6C3F1小鼠中评估了饮食中25 ppm呕吐毒素对黏膜淋巴细胞迁移途径中组成免疫器官的组织学和淋巴细胞谱的影响。给予呕吐毒素导致派尔集合淋巴结、肠系膜淋巴结和脾脏中生发中心的大小和频率显著增加。虽然呕吐毒素处理对脾脏中B细胞的百分比没有影响，但观察到派尔集合淋巴结中B细胞的百分比略有增加。在接触呕吐毒素4周、8周和12周时，派尔集合淋巴结和脾脏中IgA+细胞的百分比约为对照组的两倍，而这两个器官中IgG+细胞的百分比则下降。接触呕吐毒素会增加派尔集合淋巴结和脾脏中T细胞的百分比。呕吐毒素处理后，派尔集合淋巴结中CD4+细胞（辅助性T细胞亚群）的百分比略有增加，而脾脏中增加更为明显（30 - 50%）。相反，与对照组相比，呕吐毒素处理小鼠脾脏中CD8+细胞（细胞毒性/抑制性T细胞亚群）的百分比仅略有增加，而对派尔集合淋巴结没有影响。呕吐毒素对这两个T细胞群体的相对影响也反映在派尔集合淋巴结和脾脏中CD4+:CD8+比值的增加上。这些结果与以下假设一致，即饮食中的呕吐毒素在派尔集合淋巴结水平调节IgA反应的正常调节，这表现为黏膜和全身区室中淋巴细胞分布模式的改变。IgA+和CD4+细胞水平的显著增加分别表明产生IgA的祖细胞和辅助性T细胞亚群，它们共同作用可能有利于IgA的过度产生和血清中IgA水平的升高。
