Bonanno E, Ercoli L, Missori P, Rocchi G, Spagnoli L G
Cattedra di Anatomia Patologica, Università di Roma, Tor Vergata, Italy.
Lab Invest. 1994 Aug;71(2):308-15.
Hematopoietic microenvironment has a crucial role homing and regulating precursor cell growth both in physiologic and pathologic conditions. Fibroblast, endothelial cells, macrophages, adipocytes, and myoid cells, are the cellular component recognized in human bone marrow cultures. The presence of myoid cells in human bone marrow has been observed during fatal life, whereas during adult life, it is strictly related to pathologic conditions.
The aim of this study was to isolate a homogeneous stromal cell population. The mononuclear fraction obtained from the vertebral body of living humans was cultured without hydrocortisone and horse serum to inhibit foam cell differentiation.
The immunocytochemistry and electron microscopy characterization indicate that the cellular population we isolated had an homogeneous "myoid" differentiation. Moreover, these cells were able to support blast cell colony formation in vitro.
This method allowed the preparation of homogeneous myoid cell cultures depleted of other bone marrow stromal components. The isolation of a single stromal population is relevant in order to study the function of contractile filaments in allowing close-binding interactions with hematopoietic precursor cells.
造血微环境在生理和病理条件下对前体细胞的归巢和生长调节起着关键作用。成纤维细胞、内皮细胞、巨噬细胞、脂肪细胞和肌样细胞是人类骨髓培养物中公认的细胞成分。在生命早期即可观察到人类骨髓中存在肌样细胞，而在成年期，它与病理状况密切相关。
本研究的目的是分离出均匀的基质细胞群体。从活体人类椎体获得的单核细胞部分在无氢化可的松和马血清的条件下培养，以抑制泡沫细胞分化。
免疫细胞化学和电子显微镜表征表明，我们分离出的细胞群体具有均匀的“肌样”分化。此外，这些细胞能够在体外支持原始细胞集落形成。
该方法可制备出不含其他骨髓基质成分的均匀肌样细胞培养物。分离单一基质群体对于研究收缩丝在与造血前体细胞紧密结合相互作用中的功能具有重要意义。
