大肠杆菌mutY依赖性错配修复涉及DNA聚合酶I和短修复片段。

Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract.

作者信息

Tsai-Wu J J, Lu A L

机构信息

Department of Biological Chemistry, School of Medicine, University of Maryland at Baltimore 21201.

出版信息

Mol Gen Genet. 1994 Aug 15;244(4):444-50. doi: 10.1007/BF00286698.

DOI:10.1007/BF00286698

PMID:8078471

Abstract

Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mutY-dependent repair tract was measured by the specific incorporation of alpha-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3' side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.

摘要

在mutL背景下修复含有A/G错配的异源双链DNA需要大肠杆菌mutY基因的功能。mutY依赖的A/G错配体外修复伴随着在携带错配腺嘌呤的DNA链上进行修复DNA合成。通过将α-[32P]dCTP特异性掺入修复后DNA的不同限制性片段中来测量mutY依赖的修复片段的大小。修复片段短于12个核苷酸且长于5个核苷酸，并且定位于错配腺嘌呤的3'侧。这种修复合成由DNA聚合酶I进行。