Bauer J, Krämmer G, Knippers R
Mol Gen Genet. 1981;181(4):541-7. doi: 10.1007/BF00428750.
Heteroduplex DNA molecules were prepared in vitro using one strand of DNA carrying a point mutation and one strand of the corresponding wild-type DNA. The heteroduplex DNA was transfected into competent bacteria and the progeny genotypes in the resulting infective centers were determined. From the results we conclude that about 80% of all transfected DNA molecules are repaired before DNA replication starts. This fraction of repaired DNA is independent of the location of the mismatched nucleotide pair. However, mismatch correction occurs preferentially on the H strand of the heteroduplex DNA. The repair does not depend on a known phage coded function but requires the active bacterial genes mutU, mutH, mutS and probably mutL.
使用携带点突变的一条DNA链和相应野生型DNA的一条链在体外制备异源双链DNA分子。将异源双链DNA转染到感受态细菌中，并确定所得感染中心的子代基因型。从结果我们得出结论，在DNA复制开始之前，所有转染的DNA分子中约80%被修复。这部分修复的DNA与错配核苷酸对的位置无关。然而，错配校正优先发生在异源双链DNA的H链上。修复不依赖于已知的噬菌体编码功能，但需要活性细菌基因mutU、mutH、mutS以及可能的mutL。
