[采用手性流动相添加剂的反相高效液相色谱法对人尿中氧氟沙星对映体进行定量分析]
[Quantification of the enantiomers of ofloxacin in human urine by RP-HPLC with chiral mobile phase additive].
作者信息
Zeng S, Zhang L, Liu Z Q
机构信息
School of Pharmacy, Zhejiang Medical University, Hangzhou.
出版信息
Yao Xue Xue Bao. 1994;29(3):223-7.
PMID:8079649
Abstract
The method for determination of S-(-) and R-(+)-ofloxacin (OFLX) in human urine by RP-HPLC with a chiral mobile phase additive is described. L-phenylalanine was used as the chiral additive, Cu2+ as the ligand ion and fluorescence for detection. This chiral ligand exchange chromatography has been applied to reveal the enantioselective disposition of ofloxacin in human volunteers.
摘要
描述了一种采用手性流动相添加剂的反相高效液相色谱法测定人尿中S-(-)和R-(+)-氧氟沙星（OFLX）的方法。L-苯丙氨酸用作手性添加剂，Cu2+用作配体离子，采用荧光检测。这种手性配体交换色谱法已用于揭示氧氟沙星在人体志愿者体内的对映体选择性处置情况。
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