Kalta R R, Sharma R, Chaturvedi S C
School of Pharmacy, Devi Ahilya Vishwa Vidyalaya, Takshashila Campus, Khandwa Road (Ring Road), Indore-452 017, India.
Indian J Pharm Sci. 2008 Jul-Aug;70(4):491-4. doi: 10.4103/0250-474X.44600.
A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure was developed for simultaneous determination of nitazoxanide and ofloxacin in tablet dosage form at a single wavelength. The mobile phase used was a combination of acetonitrile:0.25M potassium dihydrogen phosphate buffer (80:20) with 0.5%v/v of triethylamine and the pH was adjusted to 2.5 by adding orthophosphoric acid. The detection of the combined dosage form was carried out at 320 nm and flow rate was set to 1ml/min. Linearity was obtained in the concentration range of 5 to 25 mug/ml of nitazoxanide and ofloxacin with correlation coefficients of 0.9987 and 0.9995, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.
建立了一种简单、精确、准确、快速且可重现的反相高效液相色谱法，用于在单一波长下同时测定片剂剂型中的硝唑尼特和氧氟沙星。所用流动相为乙腈与0.25M磷酸二氢钾缓冲液（80:20）的混合物，并含有0.5%（v/v）三乙胺，通过加入正磷酸将pH值调至2.5。在320nm处对复方制剂进行检测，流速设定为1ml/min。硝唑尼特和氧氟沙星在5至25μg/ml的浓度范围内呈线性，相关系数分别为0.9987和0.9995。分析结果进行了统计学验证，回收率研究证实了所提方法的准确性。
