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Model for in vitro investigation of bone mineralization.

作者信息

Ecsedi G G

机构信息

Cell Biology Laboratories, Research Centre of CHINOIN Pharmaceutical and Chemical Works Ltd., Budapest, Hungary.

出版信息

Agents Actions. 1994 Mar;41(1-2):84-5. doi: 10.1007/BF01986399.

DOI:10.1007/BF01986399
PMID:8079826
Abstract

An in vitro model was developed for the investigation of bone cell mineralization processes. Out-grown cells of explanted human nasal bone fragments were selected into three lineages and one of them, the osteoblast-enriched line, was partially characterized. A large number of cells of this line stained readily for alkaline phosphatase. They produced prostanoids, PGl2, PGF2 alpha and PGE2, and osteocalcin in the presence of 1,25(OH)2D3. Cells synthesized collagen and mineralized the developed extracellular matrix. This latter line seems to be appropriate as a model system to find effective compounds which increase bone calcification.

摘要

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本文引用的文献

1
The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.动物组织中脱氧核糖核酸的荧光测定，特别涉及中枢神经系统。
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Experimental studies on the cardiological effects of ipriflavone on the isolated rabbit heart and in rat and dog.异黄酮对离体兔心以及大鼠和犬心脏作用的实验研究
Arzneimittelforschung. 1981;31(6):953-8.
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Morphologic characterization of osteoblast-like cell cultures isolated from newborn rat calvaria.从新生大鼠颅骨分离的成骨细胞样细胞培养物的形态学特征
Calcif Tissue Int. 1990 Aug;47(2):92-104. doi: 10.1007/BF02555992.
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Fluorometric determination of the DNA content of cells cultured in tissue culture plates.荧光法测定组织培养板中培养细胞的DNA含量。
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Lack of bone resorption in osteosclerotic (oc/oc) mice is due to a defect in osteoclast progenitors rather than the local microenvironment provided by osteoblastic cells.
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Effect of ipriflavone and estrogen on the differentiation and proliferation of osteogenic cells.依普黄酮和雌激素对成骨细胞分化与增殖的影响。
Calcif Tissue Int. 1992;51 Suppl 1:S11-5. doi: 10.1007/BF02180243.