Higashiguchi T, Noguchi Y, Noffsinger A, Fischer J E, Hasselgren P O
Department of Surgery, University of Cincinnati Medical Center, Ohio 45267-0558.
Am J Surg. 1994 Sep;168(3):251-6. doi: 10.1016/s0002-9610(05)80197-7.
The effect of sepsis on the synthesis of endogenous and secretory proteins, including vasoactive intestinal peptide (VIP) and peptide YY (PYY), was determined in enterocytes from jejunum of rats. Sepsis was induced by cecal ligation and puncture (CLP). Control rats were sham-operated. Total endogenous and secreted protein synthesis was assessed in incubated jejunal enterocytes by measuring incorporation of 3H-phenylalanine into protein. Release of VIP and PYY into the medium of incubated enterocytes and cellular levels of the gut peptides were measured by radioimmunoassay. Sixteen hours after CLP, synthesis rates of both endogenous and secreted proteins were increased, and this effect of sepsis was most pronounced in cells from the lower parts of the villi and crypts. Enterocytes from septic rats released more VIP and PYY into the incubation medium, and approximately half of the peptides they released were newly synthesized VIP and PYY. Intracellular levels of VIP and PYY were increased as early as 4 hours after induction of sepsis. Our results suggest that sepsis stimulates the synthesis of endogenous and secretory proteins, including certain gut peptides, in small intestine mucosa. This is consistent with previous observations of increased circulating levels of VIP, PYY and other gastrointestinal hormones during sepsis. The biological significance of increased synthesis of gut peptides and other intestinal proteins during sepsis remains to be determined.
在大鼠空肠的肠上皮细胞中，测定了脓毒症对内源性和分泌性蛋白质合成的影响，这些蛋白质包括血管活性肠肽（VIP）和肽YY（PYY）。通过盲肠结扎和穿刺（CLP）诱导脓毒症。对照大鼠进行假手术。通过测量3H-苯丙氨酸掺入蛋白质的情况，评估培养的空肠肠上皮细胞中总内源性和分泌性蛋白质的合成。通过放射免疫测定法测量VIP和PYY释放到培养的肠上皮细胞培养基中的量以及肠道肽的细胞水平。CLP术后16小时，内源性和分泌性蛋白质的合成速率均增加，脓毒症的这种作用在绒毛和隐窝下部的细胞中最为明显。脓毒症大鼠的肠上皮细胞向孵育培养基中释放了更多的VIP和PYY，并且它们释放的肽中约一半是新合成的VIP和PYY。脓毒症诱导后4小时，VIP和PYY的细胞内水平就升高了。我们的结果表明，脓毒症刺激小肠黏膜内源性和分泌性蛋白质的合成，包括某些肠道肽。这与先前观察到的脓毒症期间VIP、PYY和其他胃肠激素循环水平升高一致。脓毒症期间肠道肽和其他肠道蛋白质合成增加的生物学意义仍有待确定。
