Zamir O, Hasselgren P O, Higashiguchi T, Frederick J A, Fischer J E
Department of Surgery, University of Cincinnati Medical Center, Ohio.
Am J Surg. 1992 Jan;163(1):181-4; discussion 184-5. doi: 10.1016/0002-9610(92)90273-t.
The effect of sepsis on plasma levels of various gut peptides was studied in rats. Sepsis was induced by cecal ligation and puncture (CLP); control animals underwent sham operation. Sixteen hours after CLP or sham operation, portal and systemic blood was drawn, and plasma levels of gastrin, vasoactive intestinal peptide (VIP), secretin, peptide YY (PYY), gastrin-releasing peptide (GRP), and substance P were determined by radioimmunoassay. Plasma levels of gastrin, VIP, PYY, and secretin were elevated in septic rats compared with nonseptic animals, with the highest levels noted in portal blood. There was no effect of sepsis on GRP or substance P levels. In other experiments, human recombinant interleukin 1 alpha (IL-1 alpha) or recombinant tumor necrosis factor alpha (TNF alpha) was injected intraperitoneally (300 micrograms/kg body weight in 3 divided doses over 16 hours). There was no change in plasma levels of gut peptides after IL-1 alpha injection. TNF alpha induced elevation of PYY levels in portal plasma with no change in other gut peptide levels. The results suggest that sepsis stimulates release of certain gut peptides and that TNF, but not IL-1, may be partly responsible for this response. The mechanism of the release of gut peptides and its significance in the pathophysiologic changes induced by sepsis remain to be determined.
在大鼠中研究了脓毒症对各种肠道肽血浆水平的影响。通过盲肠结扎和穿刺（CLP）诱导脓毒症；对照动物接受假手术。CLP或假手术后16小时，采集门静脉和全身血液，通过放射免疫测定法测定胃泌素、血管活性肠肽（VIP）、促胰液素、肽YY（PYY）、胃泌素释放肽（GRP）和P物质的血浆水平。与非脓毒症动物相比，脓毒症大鼠的胃泌素、VIP、PYY和促胰液素血浆水平升高，门静脉血中水平最高。脓毒症对GRP或P物质水平无影响。在其他实验中，腹腔注射人重组白细胞介素1α（IL-1α）或重组肿瘤坏死因子α（TNFα）（300微克/千克体重，分3次剂量在16小时内注射）。注射IL-1α后肠道肽血浆水平无变化。TNFα诱导门静脉血浆中PYY水平升高，其他肠道肽水平无变化。结果表明，脓毒症刺激某些肠道肽的释放，并且TNF而非IL-1可能部分参与了这种反应。肠道肽释放的机制及其在脓毒症诱导的病理生理变化中的意义仍有待确定。
