Okafo G N, Birrell H C, Greenaway M, Haran M, Camilleri P
SmithKline Beecham Pharmaceuticals, The Frythe Welwyn, Herts, United Kingdom.
Anal Biochem. 1994 Jun;219(2):201-6. doi: 10.1006/abio.1994.1258.
Studies are reported on the effect of the sodium salt of phytic acid on the resolution of peptides and proteins. Improved separation in the case of peptides is shown to be due to ion-ion pairing interactions between the positively charged peptides and the phytic acid polyanionic species. The improved peak shapes related to the proteins can be interpreted in terms of the sample preconcentration due to injection of analytes from a water medium to one of high ionic strength.
有研究报道了植酸钠对肽和蛋白质分离效果的影响。结果表明，肽分离效果的改善是由于带正电荷的肽与植酸多阴离子物种之间的离子-离子配对相互作用。与蛋白质相关的峰形改善可以通过从水介质向高离子强度介质进样导致的样品预浓缩来解释。
