Novotny M V, Cobb K A, Liu J P
Department of Chemistry, Indiana University, Bloomington 47405.
Electrophoresis. 1990 Sep;11(9):735-49. doi: 10.1002/elps.1150110911.
The current status of high-performance capillary electrophoresis as an analytical separation method for proteins, peptides and amino acids is assessed. Recent advances in suppressing the effects of electroosmotic flow and irreversible adsorption of proteins at the capillary wall are reviewed, together with procedures for optimal separations of peptides and amino acids. The detection aspects emphasize the role of laser-induced fluorescence and capillary electrophoresis/mass spectrometry in high-sensitivity measurements.
评估了高效毛细管电泳作为蛋白质、肽和氨基酸分析分离方法的当前状况。综述了抑制电渗流效应和蛋白质在毛细管壁上不可逆吸附的最新进展，以及肽和氨基酸最佳分离的程序。检测方面强调了激光诱导荧光和毛细管电泳/质谱在高灵敏度测量中的作用。
