离子色谱法作为测定人血清中总钙和总镁的潜在参考方法。

Ion chromatography as potential reference methodology for the determination of total calcium and magnesium in human serum.

作者信息

Thienpont L M, Van Nuwenborg J E, Stöckl D

机构信息

Laboratorium voor Analytische Chemie, University of Ghent, Belgium.

出版信息

Anal Chem. 1994 Jul 15;66(14):2404-8. doi: 10.1021/ac00086a029.

DOI:10.1021/ac00086a029

PMID:8080113

Abstract

The potential of ion chromatography to serve as a new reference method principle for the determination of total calcium and magnesium in human serum was investigated. Sample pretreatment consisted of acidic dilution and filtration. Detection was based on conductivity. Mean total coefficients of variation for calcium and magnesium were respectively 0.91% and 1.20% and the mean method bias was +0.06% for calcium and -0.35% for magnesium, as determined with five control materials from the Community Bureau of Reference and the National Institute of Standards and Technology. These results were comparable to those of the proposed candidate reference methods based on flame atomic absorption spectrometry. Therefore, we consider ion chromatography as a valuable reference methodology for the determination of total calcium and magnesium in human serum.

摘要

研究了离子色谱法作为测定人血清中总钙和总镁的新参考方法原理的潜力。样品预处理包括酸性稀释和过滤。检测基于电导率。使用来自欧洲标准计量局和美国国家标准与技术研究院的五种对照物质测定，钙和镁的平均总变异系数分别为0.91%和1.20%，钙的平均方法偏差为+0.06%，镁的平均方法偏差为-0.35%。这些结果与基于火焰原子吸收光谱法的候选参考方法的结果相当。因此，我们认为离子色谱法是测定人血清中总钙和总镁的一种有价值的参考方法。