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用于盘尾丝虫病诊断的封闭六边形免疫扩散法(作者译)

[The closed hexagon immunodiffusion (CHI) for the diagnosis of onchocerciasis (author's transl)].

作者信息

Neppert J

出版信息

Zentralbl Bakteriol Orig A. 1975;231(1-3):297-300.

PMID:808052
Abstract

1273 sera from microfilaria-positive and -negative persons living in an onchocerciasis-endemic area in Liberia were examined. For this purpose a modification of the Ouchterlony-test was performed and compared with other methods. In the complement-fixation test many sera showed complement inhibition without any possibility to eliminate it. There were considerably varying titers in the indirect hemagglutination test. This helped to distinguish groups with different correlations to microfilaria-findings. The closed hexagon immunodiffusion after TRAAVIK, SIEBKE and KJELDSBERG (1972) - a modified OUCHTERLONY-test - proved to be nearly as good as indirect hemagglutination. An advantage of the CHI test is that it is easy to carry out. CHI reacted in 64% of microfilaria-positive and in 25% of microfilaria-negative cases.

摘要

对利比里亚盘尾丝虫病流行地区的1273份来自微丝蚴阳性和阴性人群的血清进行了检测。为此,对奥克特洛尼试验进行了改进,并与其他方法进行了比较。在补体结合试验中,许多血清出现补体抑制现象,且无法消除。间接血凝试验中的滴度差异很大。这有助于区分与微丝蚴检测结果有不同相关性的组。经特拉维克、西贝克和凯尔兹贝格(1972年)改进的奥克特洛尼试验——封闭六边形免疫扩散试验——结果证明几乎与间接血凝试验一样好。CHI试验的一个优点是易于操作。在微丝蚴阳性病例中,CHI反应率为64%,在微丝蚴阴性病例中为25%。

相似文献

1
[The closed hexagon immunodiffusion (CHI) for the diagnosis of onchocerciasis (author's transl)].用于盘尾丝虫病诊断的封闭六边形免疫扩散法(作者译)
Zentralbl Bakteriol Orig A. 1975;231(1-3):297-300.
2
[Sera from Liberia, West Africa, cross reacting with Ascaris, hookworm and Onchocerca antigens (author's transl)].来自西非利比里亚的血清与蛔虫、钩虫和盘尾丝虫抗原发生交叉反应(作者译)
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The indirect hemagglutination test for onchocerciasis performed with blood collected on filter paper.用滤纸采集的血液进行盘尾丝虫病间接血凝试验。
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引用本文的文献

1
[Immunological diagnosis of filariosis in persons returning from tropical countries and in European and indigenous inhabitants of endemic areas (author's transl)].
Infection. 1977;5(4):219-23. doi: 10.1007/BF01640784.