Vanin A F, Mordvintcev P I, Mülsch A, Decker K
Biochemical Institute, University of Freiburg, Germany.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):571-6. doi: 10.1006/bbrc.1994.1966.
Nitric oxide (NO) production in the regenerating liver was estimated from the intensity of the electron paramagnetic resonance (e.p.r.) signal of the mononitrosyl complexes of iron and diethylthiocarbamate (DETC). Preformed complexes of intracellular non-heme Fe2+ and added DETC served as a trap for endogenously produced NO. The time-dependent changes of NO production were connected with the periodicity of liver regeneration. The first increase in NO production occurred ca. 1 h after partial hepatectomy (PHE). The second and more pronounced peak of NO production was observed about 6 h after PHE, when the hepatocytes entered the first cell cycle; it originated mainly from these cells. The following minimum of NO synthesis coincided with the maximal rate of DNA synthesis. The third gradual rise of NO production was seen at the end of the investigated period that covered the G2 + M phases, the transit from the first to the second cell cycle of the hepatocytes and the entrance of the nonparenchymal cells into proliferation.
通过铁与二乙氨基二硫代甲酸盐（DETC）的单亚硝基络合物的电子顺磁共振（e.p.r.）信号强度来估算再生肝脏中一氧化氮（NO）的生成量。细胞内非血红素Fe2+与添加的DETC预先形成的络合物可作为内源性产生的NO的捕获剂。NO生成的时间依赖性变化与肝脏再生的周期性相关。部分肝切除（PHE）后约1小时，NO生成量首次增加。PHE后约6小时，当肝细胞进入第一个细胞周期时，观察到NO生成的第二个且更为明显的峰值；它主要源自这些细胞。随后NO合成的最小值与DNA合成的最大速率相吻合。在涵盖G2 + M期、肝细胞从第一个细胞周期过渡到第二个细胞周期以及非实质细胞进入增殖阶段的研究期结束时，观察到NO生成量的第三次逐渐上升。
