Lakatos A, Sétålò J, Jobst K
Department of Clinical Chemistry, University Medical School, Pécs, Hungary.
Alcohol Alcohol. 1994 Mar;29(2):159-62.
The time-course of the reaction of H1 and total histone with glucose, acetaldehyde or both has been studied using the NBT reduction test and fluorescence. With both methods, purified H1 histone gave higher absorbance with acetaldehyde than with a 1:1 combination of glucose and acetaldehyde. For total histone, the opposite was found; a 1:1 combination of the above two aldehydes had the higher absorbance. As an explanation, the possibility of different reactivity of the amino groups with glucose and acetaldehyde is proposed. A possible simultaneous interaction between glucose, acetaldehyde and serum protein, mainly albumin, may alter the results of the diagnostic protein glycation methods, e.g. of the fructosamine test, and, therefore, also the monitoring of diabetes.
利用NBT还原试验和荧光技术研究了H1组蛋白和总组蛋白与葡萄糖、乙醛或二者的反应时间进程。使用这两种方法时，纯化的H1组蛋白与乙醛反应时的吸光度高于与葡萄糖和乙醛1:1组合反应时的吸光度。对于总组蛋白，结果则相反；上述两种醛的1:1组合具有更高的吸光度。作为一种解释，提出了氨基与葡萄糖和乙醛反应性不同的可能性。葡萄糖、乙醛与血清蛋白（主要是白蛋白）之间可能的同时相互作用可能会改变诊断蛋白质糖基化方法（如果糖胺试验）的结果，因此也会影响糖尿病的监测。
