源自阿氏利什曼原虫糖基肌醇磷脂的四种寡糖的结构
Structures of four oligosaccharides derived from the glycoinositolphospholipid of Leishmania adleri.
作者信息
Routier F, Mendonça-Previato L, Previato J O, Jones C, Wait R
机构信息
Laboratoire de Chimie Biologique, USTL, Villeneuve d'Ascq, France.
出版信息
Braz J Med Biol Res. 1994 Feb;27(2):211-7.
Glycoinositolphospholipids (GIPLs) were extracted from the trypanosomatid Leishmania adleri by hot phenol extraction and the carbohydrate moieties isolated after base cleavage. Purification of the crude oligosaccharides by high performance anion exchange (HPAE) chromatography yielded four fractions whose structures were determined by a combination of methylation analysis, fast atom bombardment (FAB) mass spectrometry and two-dimensional nuclear magnetic resonance (NMR) spectroscopy.
通过热酚提取法从锥虫利什曼原虫中提取糖基肌醇磷脂（GIPLs），并在碱裂解后分离出碳水化合物部分。通过高效阴离子交换（HPAE）色谱法对粗寡糖进行纯化，得到四个馏分，其结构通过甲基化分析、快原子轰击（FAB）质谱和二维核磁共振（NMR）光谱相结合的方法确定。
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