Jones C, Previato J O, Mendonça-Previato L, Wait R
Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, UK.
Braz J Med Biol Res. 1994 Feb;27(2):219-26.
Nuclear magnetic resonance (NMR) spectroscopy provides an extremely powerful technique to determine the structure of oligosaccharides, particularly when used in conjunction with other physical techniques such as methylation analysis and fast atom bombardment mass spectrometry (FAB-MS). This brief review describes the application of NMR to the determination of the structure of an oligosaccharide isolated from the glycophosphosphingolipid (GPS) from the monogenetic trypanosomatid Leptomonas samueli. When ambiguities arise in the NMR interpretation, the use of other data will be discussed.
核磁共振(NMR)光谱法为确定寡糖结构提供了一种极其强大的技术,特别是当它与其他物理技术如甲基化分析和快原子轰击质谱法(FAB-MS)结合使用时。这篇简短的综述描述了NMR在确定从单基因锥虫利什曼原虫的糖磷脂酰鞘氨醇(GPS)中分离出的一种寡糖结构方面的应用。当NMR解释出现模糊性时,将讨论其他数据的使用情况。