来自加州电鳐电器官的乙酰胆碱酯酶膜锚的新型糖基磷脂酰肌醇结构。
Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica.
作者信息
Mehlert A, Varon L, Silman I, Homans S W, Ferguson M A
机构信息
Department of Biochemistry, University of Dundee, Scotland, UK.
出版信息
Braz J Med Biol Res. 1994 Feb;27(2):243-8.
The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
利用核磁共振（NMR）、甲基化分析以及化学和酶促微测序技术，解析了加州电鳐电器官乙酰胆碱酯酶糖基磷脂酰肌醇（GPI）膜锚的聚糖部分结构。发现存在两种结构：Glcα1-2Manα1-2Manα1-6Manα1-4GlcNα1-6肌醇，以及Glcα1-2Manα1-2Manα1-6（GalNAcβ1-4）Manα1-4GlcNα1-6肌醇。该GPI锚结构中葡萄糖的存在是一个新特征。还表明每个分子的锚含有2.3个乙醇胺残基。
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