Portnoy D A, Smith G A, Goldfine H
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6076.
Braz J Med Biol Res. 1994 Feb;27(2):357-61.
Listeria monocytogenes is a model intracellular pathogen which escapes from a host cell vacuole, grows intracytoplasmically, and spreads cell to cell without an extracellular phase. A number of genes necessary for pathogenicity have been discovered, two of which encode phospholipases C, a PI-PLC and a broad-range PLC. Single and double mutants were constructed with in-frame deletions in one or both PLCs. Characterization of the strains indicated that the two PLCs may have overlapping function as the double mutant was 500-fold less virulent while the single mutants had a negligible effect on virulence. The role of the PLCs appears to be multifactorial as PI-PLC has a role in escaping from the initial host vacuole and the broad-range PLC appears to have a role in cell to cell spreading.
单核细胞增生李斯特菌是一种典型的细胞内病原体，它能从宿主细胞液泡中逃逸，在细胞质内生长，并在细胞间传播而不经过细胞外阶段。已经发现了许多致病性必需的基因，其中两个编码磷脂酶C，一个是PI-PLC，另一个是广谱PLC。构建了在一个或两个PLC中进行框内缺失的单突变体和双突变体。菌株的特性表明，这两种PLC可能具有重叠功能，因为双突变体的毒力降低了500倍，而单突变体对毒力的影响可忽略不计。PLC的作用似乎是多因素的，因为PI-PLC在从初始宿主液泡中逃逸方面起作用，而广谱PLC似乎在细胞间传播方面起作用。
