Deshaies Y, Dagnault A, Boivin A, Richard D
Department of Physiology, School of Medicine, Laval University, Québec, Canada.
Int J Obes Relat Metab Disord. 1994 Jun;18(6):405-11.
This study evaluated the respective and interactive effects of chronic dl-fenfluramine treatment, an anorectic serotoninergic agonist, and gonadectomy on lipoprotein lipase activity in adipose tissue and skeletal muscle. Male and female Sprague-Dawley rats were gonadectomised. These as well as intact animals were treated with dl-fenfluramine or a placebo for 28 days. Gonadectomy brought about an increase in final body weight of females (16-18%, P < 0.0001), but a decrease in that of male animals (9-13%, P < 0.001). These changes were proportional to those of food intake. The increase in body weight of gonadectomised female rats was paralleled by that of retroperitoneal adipose tissue and vastus lateralis muscle weights, whereas in male rats, gonadectomy diminished muscle weight only. Lipoprotein lipase activity was doubled (P < 0.0001) by gonadectomy in adipose tissue of female rats, but remained unaltered by the surgery in male animals. Enzyme activity in muscle was unaffected by gonadectomy in both genders. Treatment with dl-fenfluramine reduced weight gain in males and females, whether they had been gonadectomised or not. A concomitant reduction was observed in adipose tissue mass and lipoprotein lipase activity, which was reduced to 50-65% of the activity measured in placebo-treated animals (P < 0.01). The drug remained without effect on muscle weight and lipoprotein lipase activity in either gender. Thus removal of gonadal steroids had divergent effects on LPL activity with regard to gender and tissue. In addition, dl-fenfluramine treatment was followed by decreased enzyme activity in adipose tissue, but not in muscle, this pattern being independent of the nature or presence of gonadal steroids.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究评估了慢性右旋芬氟拉明治疗（一种食欲抑制性血清素能激动剂）、性腺切除术对脂肪组织和骨骼肌中脂蛋白脂肪酶活性的各自及交互作用。对雄性和雌性斯普拉格-道利大鼠进行性腺切除。这些大鼠以及未切除性腺的动物接受了28天的右旋芬氟拉明或安慰剂治疗。性腺切除术使雌性大鼠的终末体重增加（16 - 18%，P < 0.0001），但使雄性动物的终末体重下降（9 - 13%，P < 0.001）。这些变化与食物摄入量的变化成比例。去势雌性大鼠体重增加的同时，腹膜后脂肪组织和股外侧肌重量也增加，而在雄性大鼠中，性腺切除术仅使肌肉重量减少。性腺切除术使雌性大鼠脂肪组织中的脂蛋白脂肪酶活性加倍（P < 0.0001），但在雄性动物中该手术对其无影响。两性肌肉中的酶活性均不受性腺切除术影响。无论是否接受性腺切除，右旋芬氟拉明治疗均可减少雄性和雌性大鼠的体重增加。同时观察到脂肪组织质量和脂蛋白脂肪酶活性降低，降至安慰剂治疗动物所测活性的50 - 65%（P < 0.01）。该药物对两性的肌肉重量和脂蛋白脂肪酶活性均无影响。因此，去除性腺类固醇对脂蛋白脂肪酶活性的影响因性别和组织而异。此外，右旋芬氟拉明治疗后脂肪组织中的酶活性降低，但肌肉中未降低，这种模式与性腺类固醇的性质或是否存在无关。（摘要截短至250字）
