Ramanathan S, Nair N K, Mansor S M, Navaratnam V
Centre for Drug Research, Universiti Sains Malaysia, Penang.
J Chromatogr B Biomed Appl. 1994 May 13;655(2):269-73. doi: 10.1016/s0378-4347(94)80028-6.
A rapid and selective high-performance liquid chromatographic (HPLC) method for the simultaneous determination of the antifilarial drug UMF-078 (I) and its metabolites UMF-060 (II) and flubendazole (III) is described. After a simple extraction from whole blood, the compounds were determined by HPLC using a C18 Inertsil ODS-2 reversed-phase column with methanol-0.05M ammonium acetate (pH 4.0) as the mobile phase and ultraviolet detection at 291 nm. The average recoveries of I, II and III over the concentration range 20-500 ng ml-1 were 69.9 +/- 4.7, 85.6 +/- 4.4 and 85.1 +/- 6.0%, respectively. The minimum detectable concentrations in whole blood for I, II and III were 10, 7 and 7 ng ml-1, respectively. This method was found to be suitable for pharmacokinetic studies.
描述了一种快速、选择性的高效液相色谱（HPLC）方法，用于同时测定抗丝虫药物UMF-078（I）及其代谢物UMF-060（II）和氟苯达唑（III）。从全血中进行简单提取后，使用C18 Inertsil ODS-2反相柱，以甲醇-0.05M醋酸铵（pH 4.0）作为流动相，在291nm处进行紫外检测，通过HPLC测定这些化合物。在20 - 500 ng ml-1浓度范围内，I、II和III的平均回收率分别为69.9 +/- 4.7%、85.6 +/- 4.4%和85.1 +/- 6.0%。I、II和III在全血中的最低检测浓度分别为10、7和7 ng ml-1。发现该方法适用于药代动力学研究。
