采用高效液相色谱法测定全血中新型抗丝虫药UMF-058和甲苯达唑的含量。

Determination of a new antifilarial drug, UMF-058, and mebendazole in whole blood by high-performance liquid chromatography.

作者信息

Ramanathan S, Nair N K, Mansor S M, Navaratnam V

机构信息

Centre for Drug Research, Universiti Sains Malaysia Malaysia, Penang.

出版信息

J Chromatogr. 1993 Jun 2;615(2):303-7. doi: 10.1016/0378-4347(93)80345-5.

DOI:10.1016/0378-4347(93)80345-5

PMID:8335708

Abstract

A rapid and selective high-performance liquid chromatographic assay for simultaneous quantitative determination of a new antifilarial drug (UMF-058, I) and mebendazole (MBZ) is described. After a simple extraction from whole blood, both compounds were analysed using a C18 Nova Pak reversed-phase column and a mobile phase of methanol-0.05 M ammonium dihydrogenphosphate (50:50, v/v) adjusted to pH 4.0, with ultraviolet detection at 291 nm. The average recoveries of I and MBZ over a concentration range of 25-250 ng/ml were 92.0 +/- 7.7 and 84.4 +/- 4.4%, respectively. The minimum detectable concentrations in whole blood for I and MBZ were 7 and 6 ng/ml, respectively. This method was found to be suitable for pharmacokinetic studies.

摘要

本文描述了一种快速、选择性的高效液相色谱法，用于同时定量测定一种新型抗丝虫药物（UMF-058，I）和甲苯咪唑（MBZ）。从全血中进行简单萃取后，使用C18 Nova Pak反相柱和甲醇-0.05 M磷酸二氢铵（50:50，v/v）调节至pH 4.0的流动相分析这两种化合物，并在291 nm处进行紫外检测。在25-250 ng/ml的浓度范围内，I和MBZ的平均回收率分别为92.0±7.7%和84.4±4.4%。I和MBZ在全血中的最低检测浓度分别为7和6 ng/ml。该方法适用于药代动力学研究。