Van Boven M, Daenens P, Cokelaere M, Decuypere E
Katholieke Universiteit Leuven, Laboratory of Toxicology, Belgium.
J Chromatogr B Biomed Appl. 1994 May 13;655(2):281-5. doi: 10.1016/0378-4347(94)00121-9.
A solid-phase method for the extraction of simmondsin from plasma was developed along with a liquid chromatographic method for the quantitative measurement of this compound. The extraction of simmondsin is realised with activated carbon. Chromatography is performed on a 25 x 0.46 cm I.D. RP-18, 5-micron HPLC column with a water-methanol (85:15, v/v) mobile phase and ultraviolet absorbance detection at 217 nm. The limit of detection is 100 ng (using 1 ml of plasma). The linear quantitation range is 0.1-200 micrograms/ml.
开发了一种从血浆中提取西蒙德辛的固相方法以及用于该化合物定量测定的液相色谱法。西蒙德辛的提取通过活性炭实现。色谱分析在一根内径为25×0.46厘米、粒径为5微米的RP - 18高效液相色谱柱上进行，流动相为水 - 甲醇（85:15，v/v），在217纳米处进行紫外吸收检测。检测限为100纳克（使用1毫升血浆）。线性定量范围为0.1 - 200微克/毫升。
