Ponce R H, Yanagimachi R, Urch U A, Yamagata T, Ito M
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Zygote. 1993 May;1(2):163-71. doi: 10.1017/s0967199400001428.
The plasma membrane (oolemma) of the hamster egg retains the ability to fuse with spermatozoa even after exhaustive treatment with proteases and glycosidases. In contrast, when mouse oolemma is treated with proteases, the ability of eggs to fuse with spermatozoa is reduced. In the present study, similar treatments effective in reducing fusibility in the mouse were reexamined in the hamster. Of the several enzymes and treatments tested, only trypsin in Ca(2+)-free medium significantly reduced the hamster oolemma's ability to fuse with spermatozoa. This is suggestive of a cadherin-like system of binding and fusion. When hamster oolemmae were treated with the same protease regimen that reduced fusibility of mouse oolemma for mouse spermatozoa, heterologous fusion of hamster oolemmae with mouse spermatozoa was reduced, without affecting the fusion of these oolemmae with hamster spermatozoa. These data suggest that a protease-sensitive oolemma molecule is of critical importance for mouse sperm-oolemma fusion but not for hamster sperm-oolemma fusion.
仓鼠卵的质膜（卵膜）即使在用蛋白酶和糖苷酶进行彻底处理后，仍保留与精子融合的能力。相比之下，当用蛋白酶处理小鼠卵膜时，卵与精子融合的能力会降低。在本研究中，对在小鼠中有效降低融合性的类似处理在仓鼠中进行了重新检验。在所测试的几种酶和处理方法中，只有无钙培养基中的胰蛋白酶显著降低了仓鼠卵膜与精子融合的能力。这提示存在一种类似钙黏蛋白的结合和融合系统。当用降低小鼠卵膜对小鼠精子融合性的相同蛋白酶处理方案处理仓鼠卵膜时，仓鼠卵膜与小鼠精子的异源融合减少，而不影响这些卵膜与仓鼠精子的融合。这些数据表明，一种对蛋白酶敏感的卵膜分子对小鼠精子 - 卵膜融合至关重要，但对仓鼠精子 - 卵膜融合并不重要。
