Yanagimachi R, Katayose H, Killian G, Lee C N, Carrell D T, Huang T T
Department of Anatomy and Reproductive Biology, University of Hawaii.
Zygote. 1993 Nov;1(4):345-51. doi: 10.1017/s0967199400001672.
The zona pellucida of mammalian oocytes stored in highly concentrated solutions of neutral salts is known to retain its biological and biochemical properties. However, the zona may become resistant to sperm penetration as the storage period is increased. In cattle and hamsters, the penetrability of zonae of salt-stored oocytes was restored or increased by treating the oocytes with moderate heat without altering the gross morphology of the zona. Although this technique did not work for salt-stored human ova, this may have been due to the use of so-called inseminated-unfertilised ova which (1) may have been fertilised but failed to activate, or (2) were not fertilised because of functionally defective zonae.
已知储存在高浓度中性盐溶液中的哺乳动物卵母细胞透明带能保持其生物学和生化特性。然而，随着储存时间的增加，透明带可能会对精子穿透产生抗性。在牛和仓鼠中，通过对盐储存的卵母细胞进行适度加热处理，可恢复或提高其透明带的穿透性，且不会改变透明带的总体形态。尽管这项技术对盐储存的人类卵子不起作用，但这可能是由于使用了所谓的授精未受精卵，这些卵子（1）可能已经受精但未能激活，或者（2）由于功能有缺陷的透明带而未受精。
