Broders F, Girault J M, Simonneau L, Suzuki S, Thiery J P
Laboratory of Physiopathology of Development, CNRS URA 1337, Paris, France.
Cell Adhes Commun. 1993 Dec;1(3):265-77. doi: 10.3109/15419069309097259.
Cadherins are calcium-dependent adhesive glycoproteins implicated in histogenetic processes. In Xenopus laevis, the distribution of classical cadherins N, E, EP, XB and U has been determined by immunofluorescence labeling or by in situ hybridization. In this study, we report the full-length sequence of the E-cadherin cDNA. Comparison with the other cadherin sequences available indicates that Xenopus E-cadherin is as homologous to Xenopus EP-cadherin as to the chicken L-CAM and to the mammalian E-cadherin. Although Xenopus E-cadherin protein sequence exhibits many short conserved motifs present in other E-cadherins, it differs remarkably from the chicken L-CAM and the mammalian E-cadherin in its appearance after gastrulation. In situ hybridization data showed that E-cadherin transcripts are homogenously distributed in all differentiating epithelia from early tailbud to post-metamorphic stage. In contrast to mouse E-cadherin, Xenopus E-cadherin was not detected transiently in the nervous system during embryogenesis and in the post-metamorphic stages.
钙黏着蛋白是参与组织发生过程的钙依赖性黏附糖蛋白。在非洲爪蟾中，经典钙黏着蛋白N、E、EP、XB和U的分布已通过免疫荧光标记或原位杂交确定。在本研究中，我们报告了E-钙黏着蛋白cDNA的全长序列。与其他可用的钙黏着蛋白序列比较表明，非洲爪蟾E-钙黏着蛋白与非洲爪蟾EP-钙黏着蛋白的同源性与鸡L-CAM和哺乳动物E-钙黏着蛋白的同源性相同。尽管非洲爪蟾E-钙黏着蛋白的蛋白质序列表现出许多其他E-钙黏着蛋白中存在的短保守基序，但在原肠胚形成后的外观上，它与鸡L-CAM和哺乳动物E-钙黏着蛋白有显著差异。原位杂交数据表明，E-钙黏着蛋白转录本在从早期尾芽到变态后阶段的所有分化上皮中均匀分布。与小鼠E-钙黏着蛋白不同，非洲爪蟾E-钙黏着蛋白在胚胎发生期间和变态后阶段的神经系统中未被短暂检测到。
