Bowden C G, Hintz W E, Jeng R, Hubbes M, Horgen P A
Department of Botany, University of Toronto, Mississauga, Ontario, Canada.
Curr Genet. 1994 Apr;25(4):323-9. doi: 10.1007/BF00351485.
The hydrophobic protein cerato-ulmin (CU), produced by Ophiostoma ulmi, has been implicated in the pathogenicity of this fungus on elm. Primers were designed based on the nucleotide sequence deduced from the published CU amino-acid sequence, and a DNA fragment of the cu gene was amplified using the polymerase chain reaction. The amplified cu fragment was used as a hybridization probe to identify and isolate the cu gene from a genomic DNA library of an aggressive isolate of O. ulmi ( = O. novo-ulmi). The cu coding region is interrupted by two introns and encodes a 100 amino-acid prepro-CU polypeptide that is processed to a 75 amino-acid mature protein upon secretion. CU shows significant sequence similarity to hydrophobins secreted by certain other fungi.
由榆长喙壳菌（Ophiostoma ulmi）产生的疏水蛋白角质溃疡素（CU）被认为与这种真菌对榆树的致病性有关。根据已发表的CU氨基酸序列推导的核苷酸序列设计引物，并使用聚合酶链反应扩增cu基因的DNA片段。扩增的cu片段用作杂交探针，从榆树长喙壳菌（=新榆长喙壳菌）的一个强致病力分离株的基因组DNA文库中鉴定并分离cu基因。cu编码区被两个内含子打断，编码一个100个氨基酸的前原CU多肽，分泌时加工成一个75个氨基酸的成熟蛋白。CU与某些其他真菌分泌的疏水蛋白具有显著的序列相似性。
