Stringer M A, Dean R A, Sewall T C, Timberlake W E
Department of Genetics, University of Georgia, Athens 30602.
Genes Dev. 1991 Jul;5(7):1161-71. doi: 10.1101/gad.5.7.1161.
The Aspergillus nidulans CAN41 transcription unit is activated by the brlA regulatory gene early during development of the asexual reproductive apparatus, the conidiophore. Disruption of CAN41 results in a novel mutant phenotype in which conidiophore cells and spores lack an external wall layer, the rodlet layer, making them less hydrophobic than in the wild type and leading to inefficient spore dispersal. The rodletless mutation defines a new locus on chromosome III, rodA. rodA encodes a small, moderately hydrophobic polypeptide containing 8 cysteines arranged in a pattern similar to that observed in three hydrophobic cell wall proteins from the Holobasidiomycete Schizophyllum commune. We propose that the Aspergillus and Schizophyllum 8-cysteine polypeptides define a class of secreted, hydrophobic, fungal cell wall proteins that are important in the formation and function of aerial structures such as conidiophores and mushrooms.
构巢曲霉CAN41转录单元在无性繁殖器官分生孢子梗发育的早期被brlA调控基因激活。CAN41的破坏导致一种新的突变表型，其中分生孢子梗细胞和孢子缺乏外壁层，即小梗层，使得它们的疏水性低于野生型，导致孢子扩散效率低下。无小梗突变在第三条染色体上定义了一个新位点rodA。rodA编码一种小的、中度疏水的多肽，该多肽含有8个半胱氨酸，其排列模式与在担子菌裂褶菌的三种疏水细胞壁蛋白中观察到的模式相似。我们提出，曲霉和裂褶菌的8-半胱氨酸多肽定义了一类分泌型、疏水性真菌细胞壁蛋白，它们在气生结构如分生孢子梗和蘑菇的形成和功能中很重要。
