Wang F, Gao J
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1994 Apr;23(2):107-10.
After mouse pulmonary adenocarcinoma cell line LA795 was implanted in the subrenal capsule of T739 mice, the local distribution of fibronectin (FN), laminin (LN) and type IV collagen was detected by indirect immunoperoxidase and immunofluorescence staining and were observed during tumor growth at different time intervals. As the tumors progressed, it was shown that the expression of FN decreased while that of LN and type IV collagen gradually increased, their distribution patterns were also different. LN and type IV collagen were absent in the basement membrane in the tumor invasion areas. In order to verify the in vivo observations, various in vitro methods were used. We found expression of FN and LN but no expression of type IV collagen mRNA in LA795 cell line. Assay of random migration of tumor cells was employed to check the influence of FN, LN or type IV collagen on the motility of tumor cells. It was demonstrated that LN, FN, type IV collagen and in particular, LN could promote the motility of tumor cells individually. Cultured with addition of LN or FN, tumor cell colonies expanded diametrically more rapidly. The results indicated that FN, LN and type IV collagen may contribute to tumor invasion and metastasis.
将小鼠肺腺癌细胞系LA795接种于T739小鼠肾被膜下后，采用间接免疫过氧化物酶法和免疫荧光染色法检测纤维连接蛋白（FN）、层粘连蛋白（LN）和IV型胶原的局部分布，并在肿瘤生长的不同时间间隔进行观察。随着肿瘤进展，结果显示FN表达降低，而LN和IV型胶原表达逐渐增加，它们的分布模式也不同。在肿瘤侵袭区域的基底膜中不存在LN和IV型胶原。为了验证体内观察结果，采用了各种体外方法。我们发现LA795细胞系中有FN和LN表达，但无IV型胶原mRNA表达。采用肿瘤细胞随机迁移实验来检测FN、LN或IV型胶原对肿瘤细胞运动性的影响。结果表明，LN、FN、IV型胶原，尤其是LN可单独促进肿瘤细胞的运动性。添加LN或FN培养时，肿瘤细胞集落直径增长更快。结果提示，FN、LN和IV型胶原可能促进肿瘤侵袭和转移。
