Shen Q, Wang M X, Zhang T H
Department of Clinical Laboratories, Changhai Hospital.
Zhonghua Bing Li Xue Za Zhi. 1994 Apr;23(2):79-81.
Forty five specimens from human myocarditic or myopathic tissues were examined for enteroviruses by in situ hybridization with 2 biotin labelled generic enterovirus probes pCBIII/35 and pCBIII/51. Positive hybridization signals were obtained in 12 samples (26.6%). Enterovirus RNA were obtained in 5 out of these 12 samples (41.7%) by the presence of positive hybridization signals with a coxsackie B3 virus type-specific probe pCBIII/29. The positive hybridization signals were primarily localized within the cytoplasm of single or several myocytes and the myocardial interstitial tissues, and were also occasionally seen within the cytoplasm of endotheliocytes of blood vessels in the myocardium.
采用两种生物素标记的肠道病毒通用探针pCBIII/35和pCBIII/51，通过原位杂交技术，对45份取自人类心肌炎性或肌病性组织的标本进行肠道病毒检测。12份样本（26.6%）获得了阳性杂交信号。在这12份样本中的5份（41.7%），通过与柯萨奇B3病毒型特异性探针pCBIII/29杂交出现阳性信号，检测到了肠道病毒RNA。阳性杂交信号主要定位于单个或多个心肌细胞的细胞质以及心肌间质组织内，偶尔也可见于心肌血管内皮细胞的细胞质中。
