Bowles N E, Richardson P J, Olsen E G, Archard L C
Lancet. 1986 May 17;1(8490):1120-3. doi: 10.1016/s0140-6736(86)91837-4.
Full-length virus genomic RNA (7.4 kilobases; kb) was isolated from Coxsackie B2 virus purified from infected monkey kidney cells in culture. DNA complementary to 6.3 kb of virus RNA was prepared by reverse transcription and cloned in a plasmid vector. A 1.6 kb Coxsackie-B-virus-specific DNA clone derived from the conserved 3' region of the virus genome was used as a hybridisation probe to test for the presence of virus nucleic acid sequences in myocardial biopsy samples. Positive hybridisation signals, quantified by densitometry, were obtained with 9 of 17 samples from patients with histological evidence of active or healing myocarditis or dilated cardiomyopathy with inflammatory changes. No Coxsackie-B-virus-specific sequences were detected in 4 samples from patients in whom a viral aetiology was unlikely and the histological diagnosis was negative for myocarditis.
从培养的感染猴肾细胞中纯化出的柯萨奇B2病毒中分离出全长病毒基因组RNA（7.4千碱基；kb）。通过逆转录制备与6.3 kb病毒RNA互补的DNA，并克隆到质粒载体中。从病毒基因组保守的3'区域衍生出的1.6 kb柯萨奇B病毒特异性DNA克隆用作杂交探针，以检测心肌活检样本中病毒核酸序列的存在。通过密度测定法对阳性杂交信号进行定量，在17例具有活动性或愈合性心肌炎或伴有炎症改变的扩张型心肌病组织学证据的患者样本中，有9例获得了阳性信号。在4例病毒病因不太可能且心肌炎组织学诊断为阴性的患者样本中未检测到柯萨奇B病毒特异性序列。
