Easton A J, Eglin R P
Department of Biological Sciences, University of Warwick, Coventry, U.K.
J Gen Virol. 1988 Feb;69 ( Pt 2):285-91. doi: 10.1099/0022-1317-69-2-285.
A cloned cDNA probe derived from coxsackie B4 virus-infected cell RNA was shown to hybridize to the RNA of a number of different enteroviruses including coxsackie A and B viruses, echoviruses and poliovirus. The probe was used to detect virus-specific RNA sequences in cardiac tissue obtained from patients diagnosed as having a coxsackievirus infection. Virus RNA was detected using the technique of in situ hybridization in 46% (6/13) cases, but none was found in normal, control, cardiac samples. Two distinct patterns of infection were observed. The significance of these differences and the possible uses of the technique are discussed.
从柯萨奇B4病毒感染的细胞RNA中获得的一个克隆cDNA探针，被证明能与多种不同肠道病毒的RNA杂交，这些肠道病毒包括柯萨奇A组和B组病毒、埃可病毒和脊髓灰质炎病毒。该探针用于检测从被诊断为患有柯萨奇病毒感染的患者获取的心脏组织中的病毒特异性RNA序列。采用原位杂交技术在46%（6/13）的病例中检测到了病毒RNA，但在正常对照心脏样本中未发现。观察到了两种不同的感染模式。讨论了这些差异的意义以及该技术的可能用途。
