Lutz F, Kaszli F A, Bach P, Thaer A A
Institut für Pharmakologie und Toxikologie der Universität, Giessen, Germany.
Graefes Arch Clin Exp Ophthalmol. 1994 Jun;232(6):373-8. doi: 10.1007/BF00175990.
The effects of a pore-forming protein from Pseudomonas aeruginosa on the rabbit cornea were tested in vivo by measuring intraepithelial carboxyfluorescein accumulation. Carboxyfluorescein diacetate and subsequently the P. aeruginosa cytotoxin were applied by means of contact lenses with a spherical cavity on the concave surface. This allowed the application of defined concentrations of carboxyfluorescein diacetate and cytotoxin on a defined area of the corneal epithelium. Starting at 0.5 microM, cytotoxin increased the epithelial cell membrane permeability for the intracellular carboxyfluorescein within 1 min. At higher concentrations cells were shed from the epithelium. Corresponding morphological changes of the cellular structure of the corneal epithelium were observed and documented by fluorescence photomicrography. The healing process of toxified corneal epithelium appeared to be complete within 3 days. The data presented here indicate the possible role of cytotoxin-induced changes in epithelial permeability in P. aeruginosa infections. In this context, the role of soft contact lenses as a possible cytotoxin reservoir is discussed.
通过测量角膜上皮内羧基荧光素的积累，在体内测试了铜绿假单胞菌的一种成孔蛋白对兔角膜的影响。借助凹面带有球形腔的隐形眼镜施加羧基荧光素二乙酸酯，随后施加铜绿假单胞菌细胞毒素。这使得能够在角膜上皮的特定区域施加特定浓度的羧基荧光素二乙酸酯和细胞毒素。从0.5微摩尔开始，细胞毒素在1分钟内增加了细胞内羧基荧光素对上皮细胞膜的通透性。在更高浓度下，细胞从上皮脱落。通过荧光显微摄影观察并记录了角膜上皮细胞结构的相应形态变化。中毒角膜上皮的愈合过程在3天内似乎完成。此处给出的数据表明细胞毒素诱导的上皮通透性变化在铜绿假单胞菌感染中的可能作用。在这种情况下，讨论了软性隐形眼镜作为可能的细胞毒素储存库的作用。
