Thaer A, Geyer C
Klin Monbl Augenheilkd. 1985 Jul;187(1):43-8. doi: 10.1055/s-2008-1050986.
The methods presently available for examination of the corneal epithelium by use of the ophthalmic slit lamp are more or less restricted to the detection and diagnosis of significant lesions and the morphological results of pathologic processes in the epithelial layer at relatively low magnification and optical resolution. There is an increasing demand, however, for early detection of initial damaging effects to the corneal epithelium by testing simple specific reactions at cell level. Fluorescein microscopy of the intracellular turnover of fluorogenic substrates may become a valuable methodological basis for such an examination of the corneal epithelium. Orienting experimental investigations into the uptake and turnover of fluorescein-di-acetate (FDA) by the epithelial cells of the rabbit cornea after its exposure to isotonic 10(-5) molar FDA solution, using an endothelial specular microscope and an image intensifier TV camera, demonstrate that this principle permits a superior microscopic presentation not only of the cellular epithelial structure in general, but also of locally damaged areas of the epithelium immediately after their exposure to damaging chemical agents, in particular to substances affecting cell membranes.
目前利用眼科裂隙灯检查角膜上皮的方法或多或少局限于在相对低放大倍数和光学分辨率下检测和诊断上皮层明显病变以及病理过程的形态学结果。然而，通过在细胞水平测试简单的特异性反应来早期检测角膜上皮的初始损伤效应的需求日益增加。荧光素对荧光底物的细胞内周转的显微镜检查可能成为这种角膜上皮检查的有价值的方法学基础。使用内皮镜面显微镜和图像增强电视摄像机，对兔角膜上皮细胞在暴露于等渗10（-5）摩尔FDA溶液后对荧光素二乙酸酯（FDA）的摄取和周转进行定向实验研究，结果表明，这一原理不仅能以优越的显微镜呈现一般的细胞上皮结构，还能在其暴露于损伤性化学试剂，特别是影响细胞膜的物质后，立即呈现上皮局部受损区域。
